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Rapid Detection Methods for Analysis of Fungi and Mycotoxins in Agriculture Products

Author Affiliations

  • 1 Young Researchers Club, Yazd Branch, Islamic Azad University, Yazd, IRAN
  • 2 Young Researchers Club, Gorgan Branch, Islamic Azad University, Gorgan, IRAN
  • 3
  • 4

Res. J. Recent Sci., Volume 1, Issue (7), Pages 90-98, July,2 (2012)

Abstract

Many commodities and products can become contaminated with mycotoxigenic fungi and mycotoxins during harvest, storage and handling, and processing. Mycotoxins are structurally diverse, deriving from a number of biosynthetic pathways and their effect upon consumers is equally diverse ranging from acutely toxic to immunosuppressive or carcinogenic. Rapid detection and quantification of fungi and mycotoxins in agriculture products and processed food is necessary for assuring safety and quality of food. This paper provides a brief review of novel approaches and technologies for mycotoxin analysis including immunological detection, nucleic acid hybridization, polymerase chain reaction (PCR), mycotoxin biosynthetic gene, and combination assays for detection of fungi and mycotoxins. Some methods have already been commercialized while others innovative tests have been reported in the literature and have the potential for providing real-time test for fungi and mycotoxin testing. The review focuses on methods for detection of fungi and mycotoxins in agriculture products.

References

  1. Logrieco A., Mulé G., Moretti A and Bottalico., Toxigenic Fusarium species and mycotoxins associated with maize ear rot in Europe, Eur. J. Plant Path., 108, 597–609 (2002)
  2. Jones B.D. and Toal M.E., Mycotoxins in food: a UK regulatory perspective, Aspects Appl. Biol., 68, 1–9 (2003)
  3. D’Mello J.P.F., Macdonald A.M.C., Postel D., Dijksma W.T.P., Dujardin A. and Placinta C.M., Pesticide use and mycotoxin production in Fusarium and Aspergillusphytopathogens, Eur. J. Plant Path., 104, 741–51 (1998)
  4. Lee T., Han Y.K., Kim K.H., Yun S.H. and Lee Y.W., Tri13 and Tri7 determine deoxynivalenol- and nivalenol-producing chemotypes of Gibberella zeae, Appl. Env.Micro., 68, 2148–54 (2002)
  5. Varga J., Rigó K., Tóth B., Téren J. and Kozakiewicz Z., Evolutionary relationships among Aspergillus species producing economically important mycotoxins, Food Tech.Biotech., 41, 29–36 (2003a)
  6. Xu H. X., Annis S., Linz J. and Trail F., Infection and colonization of peanut pods by Aspergillus parasiticus and the expression of the aflatoxin biosynthetic gene, nor-1, in infection hyphae, Physiol. Mol. Plant Path., 56(5), 185–96 (2000)
  7. Doohan F. M., Molecular techniques for studying Fusarium ear blight of wheat, PhD thesis, Open University, UK (1998)
  8. Gilbert J. and Tekauz A., Review: recent developments in research on fusarium head blight of wheat in Canada, Can. J. Plant Path., 22, 1–8 (2000)
  9. Nelson P.E. and Plattner R.D., Fumonisins, mycotoxins produced by Fusarium species: biology, chemistry and significance, Ann. Rev. Phytopath., 31, 233– 252 (1993)
  10. Seo J.A., Proctor R.H. and Plattner R.D., Characterization of four clustered and co- regulated genes associated with fumonisin biosynthesis in Fusarium verticillioides, Fun. Gen. Biol., 34, 155–65 (2001)
  11. Bluhm B.H., Flaherty J.E., Cousin M.A. and Woloshuk C.P., Multiplex polymerase chain reaction assay for the detection of trichothecene- and fumonisin-producing species of Fusarium in cornmeal, J. Food Prot., 65, 1955–61 (2002)
  12. Danks C., Ostoja-Starzewska S., Flint J. and Banks J., The development of a lateral flow device for the discrimination of OTA producing and non-producing fungi, Aspects App.Biol., 68, 21–33 (2003)
  13. Paterson R.R.M., Archer S., Kozakiewicz Z., Lea A., Locke T. and O’Grady E., A gene probe for the patulin metabolic pathway with potential for use in patulin and novel disease control, Biocon. Sci. Tech., 10, 509–12 (2000)
  14. Boichenko L.V., Boichenko D.M., Vinokurova N.G., Reshetilova T.A. and Arinbasarov M.U., Screening for ergot alkaloid producers among microscopicfungi by means of the polymerase chain reaction, Microbiol., 70, 306–10 (2001)
  15. Miller J.D., Epidemiology of Fusarium ear diseases of cereals, in Miller J.D., and Trenholm H.L., Mycotoxins in Grain: Compounds other than Aflatoxin, St Paul MN, Egan Press, 19–36 (1994)
  16. Li S., Marquardt R.R. and Abramson D., Immunochemical detection of molds: a review, J. Food Prot., 63, 281–91 (2000)
  17. Delehanty J.B. and Ligler F.S., A microarray immunoassay for simultaneous detection of proteins and bacteria, Anal. Chem., 74, 5681–7 (2002)
  18. Kwak B.Y., Shon D.H., Kwon B.J., Kweon C.H. and Lee K.H., Detection of Aspergillus and Penicillium genera by enzyme-linked immunosorbent assay using a monoclonal antibody, J. Micro. Biotech., 11, 21–8 (2001)
  19. Brown D.W., McCormick S.P., Alexander N.J., Proctor R.H. and Desjardins A.E., Inactivation of a cytochrome P-450 is a determinant of trichothecene diversity in Fusarium species, Fun. Gen. Biol., 36, 224–33 (2002)
  20. Lévesque C.A., Molecular methods for detection of plant pathogens – what is the future? Can. J. Plant Path., 23,333–6 (2001)
  21. Banks J.N., Rizvi R.H., Barker I., Turner J.A., Rahman S. and Northway B.J., Specific monoclonal antibodies to Fusarium species and Microdochium nivale, Food Agric.Immun., 8, 249–68 (1996)
  22. Carzaniga R., Fiocco D., Bowyer P. and O’Connell R. J., Localisation of melanin in conidia of Alternaria alternata using phage display antibodies, MPMI, 15, 216–24 (2002)
  23. Konstantinova P., Bonants P.J.M., van Gent-Pelzer M.P.E.,van der Zouwen P. and van den Bulk R., Development of specific primers for detection and identification of Alternaria spp. in carrot material by PCR and comparison with blotter and plating assays, Mycol.Res., 106, 23–33 (2002)
  24. Umek R.M., Lin S.W., Vielmetter J., Terbrueggen R.H., Irvine B., Yu C.J., Kayyem J.F., Yowanto H., Blackburn G.F., Farkas D.H. and Chen Y.P., Electronic detection of nucleic acids – a versatile platform for molecular diagnostics, J. Mol. Diag., 3, 74–84 (2001)
  25. Knoll S., Mulfinger S., Niessen L. and Vogel R.F., Rapid preparation of Fusarium DNA from cereals for diagnostic PCR using sonification and an extraction kit, Plant Pathol., 51, 728–34 (2002)
  26. Parry D.W. and Nicholson P., Development of a PCR assay to detect Fusarium poae in wheat, Plant Pathol., 45,383–91 (1996)
  27. Alexander N.J., Proctor R.H., McCormick S.P. and Plattner R.D., Genetic and molecular aspects of the biosynthesis of trichothecenes by Fusarium, Cer. Res. Comm., 25, 315–20 (1997)
  28. Brown D.W., McCormick S.P., Alexander N.J., Proctor R.H. and Desjardins A.E., A genetic and biochemical approach to study trichothecene diversity in Fusariumsporotrichioides and Fusarium graminearum, Fun. Gen. Biol., 32, 121–33 (2001)
  29. Nulé G., Primed-directed enzymatic amplication of DNA with a thermostable DNA polymerase, Appl. Env. Micro., 63, 1843–6 (2001)
  30. Nicholson P., Simpson D.R., Wilson A.H., Chandler E. and Thomsett M., Detection and differentiation of trichothecene and enniatin producing Fusarium species on small-grain cereals, European Journal of Plant Pathology.,59, 29-43(2004)
  31. Carter J.P., Rezanoor H.N., Holden D., Desjardins A.E., Plattner R.D. and Nicholson P., Variation in pathogenicity associated with the genetic diversity of Fusarium graminearum, Eur. J. Plant Path., 108, 573–83 (2002)
  32. Schmidt H., Ehrmann M., Vogel R.F., Taniwaki M.H. and Niessen L., Molecular typing of Aspergillus ochraceus and construction of species specific SCAR-primers based on AFLP, Syst. Appl. Micro., 26, 138–46 (2003)
  33. O’Brian G.R., Fakhoury A.M., and Payne G.A., Identification of genes differentially expressed during aflatoxin biosynthesis in Aspergillus flavus and Aspergillus parasiticus, Fun. Gen. Biol., 39, 118–27 (2003)
  34. Amoah B.K., MacDonald M.V., Rezanoor H.N. and Nicholson P., The use of random amplified polymorphic DNA technique to identify mating groups in the Fusarium section Liseola, Plant Pathol., 45, 115–25 (1996)
  35. Doohan F.M., Weston G., Rezanoor H.N., Parry D.W. and Nicholson P., Development and use of a reversen transcription-PCR assay to study expression of Tri5 by Fusarium species in vitro and in planta, Appl. Env. Micro., 65, 3850–4 (1999)
  36. O’Donnell K., Kistler H.C., Tacke B.K. and Casper H.H., Gene genealogies reveal global phylogeographic structure and reproductive isolation among lineages of Fusariumgraminearum, the fungus causing wheat scab, PNAS., 97,7905–10 (2000)
  37. Niller J.D., Development of a PCR based assay for rapid and reliable identification of pathogenic Fusaria, Plant Breed, 19–36 (2000)
  38. Edwards S.G., O’Callaghan J. and Dobson A.D.W., PCRbased detection and quantification of mycotoxigenic fungi, Mycol. Res., 106, 1005–25 (2002)
  39. Sidhu G.S., Mycotoxin genetics and gene clusters, Eur. J. Plant Path., 108, 705–11 (2002)
  40. Trapp S.C., Hohn T.M., McCormick S. and Jarvis B.B., Characterization of the gene cluster for biosynthesis of macrocyclic trichothecene in Myrothecium roridum, MGG., 257, 421–32 (1998)
  41. Lee T., Oh D.W., Kim H.S., Lee J., Kim Y.H., Yun S.H. and Lee Y.W., Identification of deoxynivalenol and nivalenol-producing chemotypes of Gibberella zeae by using PCR, Appl. Env. Micro., 67, 2966–72 (2001)
  42. Kimura M., Anzai H. and Yamaguchi I., Microbial toxins in plant-pathogen interactions: biosynthesis, resistance mechanisms, and significance, J. Gen. Appl. Micro., 47,149–60 (2001)
  43. Kondo T., Sakurada M., Okamato S., Ono M., Tsukigi H., Suzuki A., Nagasawa H. and Sakuda S., Effects of Aflastatin A, an inhibitor of aflatoxin production, on aflatoxin biosynthetic pathway and glucose metabolism in Aspergillus parasiticus, J. Antibiotics, 54, 650–7 (2001)
  44. Chandler E.A., Simpson D.R., Thomsett M.A. and Nicholson P., Development of PCR assays to Tri7 and Tri13 and characterisation of chemotypes of Fusarium graminearum, Fusarium culmorum and Fusarium cereali, Physiol. Mol. Pl. Path., 62, 355–67 (2003)
  45. Proctor R. H., Desjardins A. E., Plattner R. D. and Hohn T. M., A polyketide synthase gene required for biosynthesis of fumonisin mycotoxins in Gibberella fujijuroi mating population A, Fun. Genet. Biol., 27, 100–12 (1999)
  46. Moreno O.J. and Kang M.S., Aflatoxins in maize: The problem and genetic solutions, Plant Breed., 118, 1–16 (1999)
  47. Varga J., Rigó K., Molnár J., Tóth B., Szencz S., Téren J. and Kozakiewicz Z., Mycotoxin production and evolutionary relationships among species of Aspergillussection Clavati, Antonie van Leeuwen., 81, 191–200 (2003b)
  48. Paterson R.R.M., Kozakiewicz Z., Locke T., Brayford D. and Jones S.C.B., Novel use of the isoepoxydon dehydrogenase gene probe for the patulin metabolic pathway and chromatography to test penicillia isolates from apple producion systems for the potential to contaminate apple juice with patulin, Food Micro., 20, 359–64 (2003)
  49. Tudzynski P., Correia T. and Keller U., Biotechnology and genetics of ergot alkaloids, Appl. Micro. Biotech., 57, 593–605 (2001)
  50. Schaad N.W. and Frederick R.D., Real-time PCR and its application for rapid plant disease diagnostics, Can. J. Plant Path., 24, 250–8 (2002)
  51. Nicholson P., Simpson D.R., Weston G., Rezanoor H.N., Lees A.K., Parry D.W. and Joyce D., Detection and quantification of Fusarium culmorum and Fusarium graminearum in cereals using PCR assays, Physiol. Mol. Plant Path., 53, 17–37 (1998)
  52. Schilling A.G., Möller E.M. and Geiger H.H., Polymerase chain reaction-based assays for species-specific detection of Fusarium culmorum, F. graminearum and F. avenaceum, Phytopathology., 86, 515–22 (1996)
  53. Proctor R.H., Brown D.W., Plattner R.D. and Desjardins A.E., Co-expression of 15 contigous genes delineates a fumonisin biosynthetic cluster in Gibberella moniliformis, Fun. Genet. Biol., 38, 237–49 (2003)
  54. Meek I.B., Peplow A.W., Ake C., Phillips T.D. and Beremand M.N., Tri1 encodes the cytochrome P450 monooxygenase for c-8 hydroxylation during trichothecene biosynthesis in Fusarium sporotrichioides and resides upstream of another new Tri gene, Appl. Env.Micro., 69,1607–13 (2003)